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| 一种获取细胞免疫信息的图像分析方法 |
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杨大刚1, 窦万春1, 张习文2, 蔡士杰1
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1.南京大学,计算机软件新技术国家重点实验室,江苏,南京,210093;2.中国科学院,软件研究研究所,人机交互技术与智能信息处理实验室,北京,100080
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| 摘要: |
| 细胞免疫信息在医学和生物学上有着广泛的用途.通过分析彩色免疫荧光图像获此信息.给出一种动态种子值像素生长算法,在均匀颜色空间中提取荧光区域;提出了椭圆匹配方法,从得到的区域中提取细胞;提出了预计算和离散化的方法,计算并分解细胞颜色,以得到免疫信息.讨论了提出的图像分析方法对细胞免疫信息获取的影响.实验表明,获得的免疫信息满足实际医学分析的需要. |
| 关键词: 颜色分解 椭圆匹配 区域提取 细胞提取 图像分析 |
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| 基金项目:Supported by the National Natural Science Foundation of China under Grant No.60303025(国家自然科学基金);the Natural Science Foundation of Jiangsu Province of China under Grant No.BK2004411(江苏省自然科学基金) |
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| An Image Analysis Method for Acquiring Cell's Immunological Information |
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YANG Da-Gang,DOU Wan-Chun,ZHANG Xi-Wen,CAI Shi-Jie
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| Abstract: |
| Cell’s immunological information is acquired through analyzing color immunofluorescence image. A pixel growing algorithm with dynamic seed values is proposed to extract fluorescent regions in the CIE (L*, a*, b*) uniform color space. Ellipse matching method is proposed to extract cells from the obtained fluorescent regions. Finally antigen information is acquired through computing and decomposing color of the cell with the pre-computation and discretization method. The methods proposed for the acquirement of cell’s immmunological information are appropriately evaluated. The experiment indicates that acquired the immunological information meets practical medicine analysis. |
| Key words: color decomposition ellipse matching region extraction cell extraction image analysis |
